Regulation of invertase of Actinomyces viscosus

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Regulation of invertase of Actinomyces viscosus.

The regulation of diethylaminoethyl-partially purified invertase (EC 3.2.1.26; beta-D-fructofuranoside fructohydrolase) from the 37,000 X g-soluble intracellular fluid of Actinomyces viscosus serotype 2 strain M-100 was studied. Glycolytic intermediates, mono-, di-, and triphosphate nucleotides, inorganic phosphate, and various divalent cations were tested for regulatory effects. Fructose-6-pho...

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Identification , Separation , and Preliminary Characterization of Invertase and , - Galactosidase in Actinomyces viscosus

The initial step of disaccharide dissimilation by Actinomyces viscosus serotype 2 strain M-100 was studied. Sucrase activity was found in the 3,000 x g particulate fraction and the 37,000 x g soluble fraction of the cells, whereas lactase activity was found almost exclusively in the 37,000 x g soluble fraction. Neither sucrase nor lactase activity was appreciable in the culture liquor. Sucrose ...

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Identification, separation, and preliminary characterization of invertase and beta-galactosidase in Actinomyces viscosus.

The initial step of disaccharide dissimilation by Actinomyces viscosus serotype 2 strain M-100 was studied. Sucrase activity was found in the 3,000 X g particulate fraction and the 37,000 X g soluble fraction of the cells, whereas lactase activity was found almost exclusively in the 37,000 X g soluble fraction. Neither sucrase nor lactase activity was appreciable in the culture liquor. Sucrose ...

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Surface fibrils (fimbriae) of Actinomyces viscosus T14V.

Surface antigens of Actinomyces viscosus T14V were released from cell walls by digestion with lysozyme. These were separated by ion-exchange and gel filtration chromatography into fractions rich in carbohydrate or protein. The former contained a polysaccharide high in 6-deoxytalose, along with a peptide fragment from the cell wall. In the protein-rich fractions, material of high molecular weigh...

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Mannose-contaminating agglutinin for Actinomyces viscosus and Actinomyces naeslundii.

Rapid agglutination of Actinomyces viscosus and Actinomyces naeslundii cells by D-mannose solutions was observed during studies of their attachment to mammalian cells in vitro. The specificity of the agglutination reaction was studied by slide agglutination tests and by measuring the rate of decrease in optical density of bacterial phosphate buffer suspensions caused by the setting of bacterial...

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 1977

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.17.3.510-512.1977